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If set on 2 then 3DM will only show positions in the network for which at least two different mutations have been published that had an effect on specificity. This is to ensure no false positive positives are included. In protein families for which a lot of mutations are available it is usually smart to leave this at at least two. If there is not so much data available it might be better to set it on 1 to get reliable E-scores. |
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