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Select the "panel design" option in 3DM. First we will divide the super-family based on sequence motifs. Because we want maximize the specificity range in the panel, we will use the "specificity hotspot" basket you have generated in question 3130. This way all sequences with the same motif at our specificity hotspots (thus will likely have the same specificity) will be in one group. Use the "add hotspots" button to select the hotspot basket you made in Q30 (it should contain 9 positions).
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- Select the "Manual" tab in the left box and type V117C. To add this mutation to the experiment, simply drag this mutation into the right box.
- Save the mutations by clicking "save" in the right box.
- Click on "Sequences" on the left.
- At "Maximum # of mutations per sequence" select 4 and select "fill up each sequence to contain maximum # of mutations". This will ensure that all sequences will have 4 mutations. If you don't use this option then sequences can have single, double, and triple mutations. Be sure to check the option “create demo measurement data”.
- Select 96 sequences to generate and set the minimum number of observations on to 2 and click on "convolute mutations".
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